Isolation and Subfractionation of Plasma ... - Biocyclopedia
Isolation and Subfractionation of Plasma Membranes to Purify Caveolae Separately from Lipid Rafts I. INTRODUCTION Cellular membranes contain distinct microdomains with unique molecular topographies, including caveolae and lipid rafts (Fig. 1).Caveolae are specialized flask-shaped invaginated microdomains located on the cell surface membrane of many cell types …
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double distilled water (ddH 2 O), 0.5% Triton X-100, 56.3 mg mL‒1 α-pinene, or 15 mM H 2 O 2, were inoculated on fungal mats of B. cinerea and incubated at 25 °C in three replicates and photographed periodically. Subsequently, the nematodes were isolated from the plates by the Baermann funnel method and counted under a stereomicroscope (Leica
Read More...Functional Analysis of Genes GlaDFR1 and GlaDFR2 …
The ddH 2 O and WT tobacco plants were used as blank and negative controls, respectively. The 25 μL PCR reaction contained 1 μL template, 1 μL of each of the GlaDFRs forward and GlaDFRs reverse primers, 12.5 μL 2x EasyTaq® PCR SuperMix, and 9.5 μL ddH 2 O. The PCR procedures were as follows: predenaturation at 94°C for 3 min, followed by ...
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Homogenize cells in an ice-cold Dounce tissue grinder. Perform the task with the grinder on ice. We recommend 30-50 passes with the grinder; however, efficient homogenization may depend on the cell type. Notes: a) To check the efficiency of homogenization, pipette 2-3 μl of the homogenized suspension onto a coverslip and observe under a ...
Read More...Extraction of polysaccharides from Amaranthus ... - PeerJ
Background Amaranthus hybridus L. is an annual herb that belongs to the Amaranthceae family, a type of multi-purpose grain, vegetable and feed crop that has received considerable attention due to its great economic value. However, the composition of polysaccharides from A. hybridus has rarely been previously reported. Methods In this study, …
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ground in tissue grinder (Jingxin, China). Frozen powder was added with 750μl buer (methanol: ddH 2 O: acetic acid (80:19:1) (V: V: V)) together with 7.5ng D6-ABA (internal standard) (Sigma, USA) and was extracted over-night at 4 °C and then centrifuged for 10 min at 13000 rpm at 4°C. e supernatant was then transferred to a new
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Read More...(PDF) Effects of processing method and age of leaves on ...
Using Japanese-style green tea-processing of young leaves, and black tea-processing of mature (BTP-M) coffee leaves, produced contrasting effects on phenolic content, and associated antioxidant ...
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Gelatin microparticle preparation. Gelatin microparticles were fabricated from acidic gelatin (Nitta Gelatin Inc., Osaka, Japan) following established procedures 20.Briefly, a gelatin solution was prepared by dissolving 5 g of gelatin in 45 ml of distilled, deionized water (ddH 2 O) at 60°C. Then, this solution was added dropwise to 250 ml of chilled olive oil while stirring at …
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Read More...Functional Analysis of Genes GlaDFR1 and GlaDFR2 …
The ddH 2 O and WT tobacco plants were used as blank and negative controls, respectively. The 25 μL PCR reaction contained 1 μL template, 1 μL of each of the GlaDFRs forward and GlaDFRs reverse primers, 12.5 μL 2x EasyTaq® PCR SuperMix, and 9.5 μL ddH 2 …
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Read More...Extraction of polysaccharides from Amaranthus ... - PeerJ
Background Amaranthus hybridus L. is an annual herb that belongs to the Amaranthceae family, a type of multi-purpose grain, vegetable and feed crop that has received considerable attention due to its great economic value. …
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Read More...Isolation and Subfractionation of Plasma ... - Biocyclopedia
Isolation and Subfractionation of Plasma Membranes to Purify Caveolae Separately from Lipid Rafts I. INTRODUCTION Cellular membranes contain distinct microdomains with unique molecular topographies, including caveolae and lipid …
Read More...Effects of processing method and age of leaves on ...
Table 1 shows the quantities, (with Supplemental Fig. 1 showing HPLC profiles) of caffeine, trigonelline, 3-CQA, 5-CQA, mangiferin, isomangiferin, rutin, 3,4-diCQA and 3,5-diCQA in various processed coffee leaf extracts. Although 5-CQA was the most abundant chlorogenic acid present in coffee leaves, an interaction with the processing method and the age of leaves …
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